SrasV1, Main, Exploration, bibRecord, 003F94

Amino acids 15-28 in the ectodomain of SARS coronavirus 3a protein induces neutralizing antibodies.

Identifieur interne : 003F94 ( Main/Exploration ); précédent : 003F93; suivant : 003F95

Amino acids 15-28 in the ectodomain of SARS coronavirus 3a protein induces neutralizing antibodies.

Auteurs : Sara Akerström [Suède] ; Yee-Joo Tan ; Ali Mirazimi

Source :

FEBS letters [ 0014-5793 ] ; 2006.

RBID : pubmed:16781713

Descripteurs français

KwdFr :
- Acides aminés (immunologie), Animaux, Anticorps antiviraux (immunologie), Cellules COS, Humains, Lapins, Protéines virales (), Protéines virales (immunologie), Sites de fixation des anticorps, Structure tertiaire des protéines, Tests de neutralisation, Virus du SRAS (immunologie), Épitopes (), Épitopes (immunologie).
MESH :
- immunologie : Acides aminés, Anticorps antiviraux, Protéines virales, Virus du SRAS, Épitopes.
- Animaux, Cellules COS, Humains, Lapins, Protéines virales, Sites de fixation des anticorps, Structure tertiaire des protéines, Tests de neutralisation, Épitopes.

English descriptors

KwdEn :
- Amino Acids (immunology), Animals, Antibodies, Viral (immunology), Binding Sites, Antibody, COS Cells, Chlorocebus aethiops, Epitopes (chemistry), Epitopes (immunology), Humans, Neutralization Tests, Protein Structure, Tertiary, Rabbits, SARS Virus (immunology), Viral Proteins (chemistry), Viral Proteins (immunology).
MESH :
- chemical , chemistry : Epitopes, Viral Proteins.
- chemical , immunology : Amino Acids, Antibodies, Viral, Epitopes, Viral Proteins.
- immunology : SARS Virus.
- Animals, Binding Sites, Antibody, COS Cells, Chlorocebus aethiops, Humans, Neutralization Tests, Protein Structure, Tertiary, Rabbits.

Abstract

A synthetic peptide corresponding to amino acids (aa) 15-28 of the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein was used to raise polyclonal antibodies in rabbits. This anti-3a N-terminal antibody could detect 3a protein in infected cells, as did an anti-3a C-terminal antibody previously described. The latter targeted the C-terminal cytoplasmic domain of 3a (aa 134-274). The anti-3a N-terminal antibody could detect intracellular 3a as well as 3a expressed on the cell surface. Interestingly, only the anti-3a N-terminal antibody can inhibit SARS-CoV propagation in Vero E6 culture although the binding affinity of the anti-3a N-terminal antibody was lower than the anti-3a C-terminal antibody.

DOI: 10.1016/j.febslet.2006.06.002
PubMed: 16781713

Affiliations:

Suède

Le document en format XML

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<term>Chlorocebus aethiops</term>
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<term>Epitopes (immunology)</term>
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<term>Humains</term>
<term>Lapins</term>
<term>Protéines virales ()</term>
<term>Protéines virales (immunologie)</term>
<term>Sites de fixation des anticorps</term>
<term>Structure tertiaire des protéines</term>
<term>Tests de neutralisation</term>
<term>Virus du SRAS (immunologie)</term>
<term>Épitopes ()</term>
<term>Épitopes (immunologie)</term>
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<term>Protéines virales</term>
<term>Sites de fixation des anticorps</term>
<term>Structure tertiaire des protéines</term>
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<front><div type="abstract" xml:lang="en">A synthetic peptide corresponding to amino acids (aa) 15-28 of the severe acute respiratory syndrome coronavirus (SARS-CoV) 3a protein was used to raise polyclonal antibodies in rabbits. This anti-3a N-terminal antibody could detect 3a protein in infected cells, as did an anti-3a C-terminal antibody previously described. The latter targeted the C-terminal cytoplasmic domain of 3a (aa 134-274). The anti-3a N-terminal antibody could detect intracellular 3a as well as 3a expressed on the cell surface. Interestingly, only the anti-3a N-terminal antibody can inhibit SARS-CoV propagation in Vero E6 culture although the binding affinity of the anti-3a N-terminal antibody was lower than the anti-3a C-terminal antibody.</div>
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<country name="Suède"><noRegion><name sortKey="Akerstrom, Sara" sort="Akerstrom, Sara" uniqKey="Akerstrom S" first="Sara" last="Akerström">Sara Akerström</name>
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Serveur d'exploration SRAS

Amino acids 15-28 in the ectodomain of SARS coronavirus 3a protein induces neutralizing antibodies.

Amino acids 15-28 in the ectodomain of SARS coronavirus 3a protein induces neutralizing antibodies.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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